Faculty and Staff

Education

Ph.D. Plant Molecular and Cellular Biology Program, University of Florida

M.S. Botany, National Taiwan University

B.S. Botany, National Taiwan University

Professional Experience

Yuan Ze University Chairman, International Bachelor Program in Engineering

Yuan Ze University Associate professor

Yuan Ze University Assistant professor

Academia Sinica Postdoctoral fellow

University of Florida Postdoctoral Research Scientist

Research Area

1. Engineering protein expression system in rice cells for valuable medical protein production

2. DNA detection biosensor for GMO

3. Develop plant tissue culture systems for snow lotus

Journal Papers

Publication:

1. Chi, Y.-H. and Huang, L.-F. (2022) Current strategies to improve yield of recombinant protein production in rice suspension cells. Processes. 10(6):1120. https://doi.org/10.3390/pr10061120 (SCI, Impact Factor: 3.352).

2. Nguyen, T.M., Lu, C.A., Huang, L.F.* (2022) Applications of CRISPR/Cas9 in a rice protein expression system via an intron-targeted insertion approach. Plant Science, 315:111132. https://doi.org/10.1016/j.plantsci.2021.111132 (SCI, Impact Factor: 4.729).

3. Wu, C.L., Lin, L.F., Hsu, H.C., Huang, L.F., Hsiao, C.D., Chou, M.L. (2021) Saussurea involucrata (Snow Lotus) ICE1 and ICE2 orthologues involved in regulating cold stress tolerance in transgenic Arabidopsis. Int. J. Mol. Sci., 22, 10850. https://doi.org/10.3390/ijms221910850 (SCI, Impact Factor: 5.924).

4. Sinaga, D.S., Ho, S.L, Lu. C.A., Yu, S.M., Huang, L.F.* (2021) Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells. Plant Methods, 25(17):99. doi: 10.1186/s13007-021-00799-2 (SCI, Impact Factor: 4.993).

5. Huang, L.F.*, Sinaga, D.S., Tan, C.C., Hsieh, S.J.M., Huang, C.H. (2021) Expression of recombinant human octamer-binding transcription factor 4 in rice suspension cells. Int. J. Mol. Sci., 22: 1409. https://doi.org/10.3390/ijms22031409 (SCI, Impact Factor: 5.924).

6. Chuang, J.N., Diao, P.Y., Huang, W.S., Huang, L.F., Senapati, S., Chang, H.C. and Sun, Y.M.* (2020) Novel homogeneous anion exchange membranes for reproducible and sensitive nucleic acid detection via current–voltage characteristic measurement, ACS Applied Materials and Interfaces, 49: 54459–54472 (SCI, Impact Factor: 9.229).

7. Huang, L.F.*, Liu, Y.K., Su, S.-C., Lai, C.-C., Wu, C.-R., Chao, T.-J. Yang, Y.H. (2020) Genetic engineering of transitory starch accumulation by knockdown of OsSEX4 in rice plants for enhanced bioethanol production, Biotechnol Bioeng, 117: 933-944 (SCI, Impact Factor: 4.53).

8. Liu, Y.K., Lu, C.W., Chang, J.Y., Lu, C.F., Tan, C.C. and Huang, L.F.* (2018) Optimization of the culture medium for recombinant protein production under the control of the αAmy3 promoter in a rice suspension-cultured cell expression system, Plant Cell, Tissue and Organ Culture, 132: 383–391. (SCI, Impact Factor: 2.711).

9. Huang, L.F., Lin, K.H., He, S.L., Chen, J.L., Jiang, J.Z., Chen, B.H., Hou, Y.S., Chen, R.S., Hong, C.Y. and Ho, S.H.* (2016) Multiple patterns of regulation and overexpression of a ribonuclease-like pathogenesis-related protein gene, OsPR10a, conferring disease resistance in rice and Arabidopsis. PLoS ONE, 11(6): e0156414. doi:10.1371/journal.pone.0156414. (SCI, Impact Factor: 3.240).

10. Huang, C.K., Shen, Y.L., Huang, L.F., Wu, S.J., Yeh, C.H., Lu, C.A.* (2016) The DEAD-Box RNA Helicase AtRH7/PRH75 Participates in Pre-rRNA Processing, Plant Development and Cold Tolerance in Arabidopsis. Plant Cell Physiol., 57(1):174-91. doi: 10.1093/pcp/pcv188. (SCI, Impact Factor: 4.927).

11. Huang, L.F.*, Tan, C.C., Yeh, J.F., Liu, H.Y., Liu, Y.K., Ho, S.L., Lu, C.A. (2015) Efficient secretion of recombinant proteins from rice suspension cultured cells modulated by the choice of signal Peptide. PLoS ONE, 10(10): e0140812. doi:10.1371/journal.pone.0140812 (SCI, Impact Factor: 3.240).

12. Huang, L.F.*; Lin, J.Y.; Pan, K.Y.; Huang, C.K.; Chu, Y.K. (2015) Overexpressing Ferredoxins in Chlamydomonas reinhardtii increase starch and oil yields and enhance electric power production in a photo microbial fuel cell. Int. J. Mol. Sci., 16: 19308-19325, Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581298/ (SCI, Impact Factor: 5.924).

13. Huang, C.K., Lo, P.C., Huang, L.F., Wu, S.J., Yeh, C.H., Lu, C.A.* (2015) A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis. Plant Mol Biol, 88(3):269-86. doi: 10.1007/s11103-015-0321-2. (SCI, Impact Factor: 4.076).

14. Liu, Y.K., Li, Y.T., Lu, C.F., Huang, L.F.* (2015) Enhancement of recombinant human serum albumin in transgenic rice cell culture system by cultivation strategy. New Biotechnology, 32(3): 328-334 (SCI, Impact Factor: 5.079).

15. Lin. Y.H., Huang. L.F., Hase, T., Huang, H. E., Feng, T.Y. (2015) Expression of plant ferredoxin-like protein (PFLP) enhances tolerance to heat stress in Arabidopsis thaliana. New Biotechnolgy, 32(2): 235-242 (SCI, Impact Factor: 5.079).

16. Chou, W.L., Huang, L.F., Fang, J.C., Yeh, C.H., Hong, C.Y., Wu, S.J., Lu, C.A. (2014) Divergence of the expression and subcellular localization of CCR4‑associated factor 1 (CAF1) deadenylase proteins in Oryza sativa. Plant Mol Biol, 85(4-5): 443-458 (SCI, Impact Factor: 4.076).

17. Chen, W.C., Lai, Y.A., Lin, Y.C., Ma, J.W., Huang, L.F., Yang, N.S., Ho, C.T., Kuo, S.C., Way, T.D. (2013) Curcumin suppresses Doxorubicin-induced epithelial-mesenchymal transition via the inhibition of TGF-β and PI3K/AKT signaling pathways in triple-negative breast cancer cells. Journal of Agricultural and Food Chemistry, 61(48): 11817~11824 (SCI, Impact factor 5.279).

18. Chen. C.C., Chen, Y.; Hsi, Y.T.; Chang, C.S.; Huang, L.F., Ho, C.T., Way T.D., Kao, J.Y. (2013) Chemical constituents and anticancer activity of Curcuma zedoaria Roscoe essential oil against non-small cell lung carcinoma cells in vitro and in vivo. Journal of Agricultural and Food Chemistry, 61(47): 11418~11427 (SCI, Impact factor 5.279).

19. Lin, Y.H., Pan, K.Y., Hung, C.H., Huang, H.E., Chen, C.L., Feng, T.Y., Huang, L.F.* (2013) Over-expression of Ferredoxin, PETF, enhances tolerance to heat stress in Chlamydomonas reinhardtii. Int. J. Mol. Sci.,14(10) 20913-20929. Link: http://www.mdpi.com/1422-0067/14/10/20913 (SCI, Impact factor: 5.924).

20. Kuo, Y.C., Tan, C.C., Ku, J.T., Hsu, W.C., Su, S.C., Lu, C.A., Huang, L.F.* (2013) Improving Pharmaceutical Protein Production in Oryza sativa. Int. J. Mol. Sci., 14: 8719-8739. Link: http://www.mdpi.com/1422-0067/14/5/8719 (SCI, Impact factor: 5.924).

21. Ho, S.L., Huang, L.F., Lu, C.A., Wang, C.C., Chen, J and Yu, S.M. (2013) Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings. Plant Mol Biol, 81:347-361 (equal contribution as the first author, SCI, Impact Factor: 4.076).

22. Liu, Y.K., Huang, L.F., Ho, S.L., Liao, C.Y., Liu, H.Y., Lai, Y.H., Yu, S.M. and Lu, C.A. (2012) Production of mouse granulocyte-macrophage colony stimulating factor by Gateway technology and transgenic rice cell culture. Biotechnology & Bioengineering, 109:1239-1247 (SCI, Impact Factor: 4.53).

23. Lo, Y.C., Huang, L.F., Cheng, C.L., Chen, J. and Chang, J.S. (2011) Using a starch-rich mutant of Arabidopsis thaliana as feedstock for fermentative hydrogen production. Bioresource Technology, 102:8543-8546. (SCI, Impact factor: 9.642).

24. Huang, C.K., Huang, L.F., Huang, J.J., Wu, S.J., Yeh, C.H., Lu, C.A. (2010) A DEAD-box protein, AtRH36, is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis. Plant and Cell Physiology, 51:694-706 (SCI, Impact factor: 4.927).

25. Lin, H.H., Huang, L.F., Su, H.C., Jeng. S.T. (2009) Effects of the multiple polyadenylation signal AAUAAA on mRNA 3'-end formation and gene expression. Planta, 230:699-712 (equal contribution as the first author, SCI, Impact Factor: 4.116).

26. Lu, C.A., Lin, C.C., Lee, K.W., Chen, J.L., Huang, L.F., Ho, S.L., Liu, H.J., Hsing, Y.I. and Yu, S.M. (2007) The SnRK1A protein kinase plays a key role in sugar signaling during germination and seedling growth of Rice. Plant Cell, 19: 2484-2499 (SCI, Impact Factor: 11.277).

27. Huang, L.F., Bocock, P.N., Davis , J.M. and Koch, K.E. (2007) Invertase regulation: a “suite” of transcriptional and post-transcriptional mechanisms. Functional Plant Biology, 34:499-507 (SCI, Impact Factor: 3.101).

28. Kohorn, B.D., Kobayashi, M., Johansen, S., Riese, J., Huang, L.F., Koch, K., Fu, S., Dotson, A. and Byers, N. (2006) An Arabidopsis cell wall-associated kinase required for invertase activity and cell growth. Plant Journal, 46:307-316 (SCI, Impact Factor: 6.486).

29. Huang, L.F., Liu, Y.K., Lu, C.A., Hsieh, S.L. and Yu, S.M. (2005) Production of human serum albumin by sugar starvation induced promoter and rice cell culture. Transgenic Research, 14:569-581 (SCI, Impact Factor: 2.788).

30. Chiang, C.M., Yeh, F.S., Huang, L.F., Tseng, T.H., Chung M.C., Wang C.S., Lur, H.S., Shaw, J.F. and Yu, S.M. (2005) Expression of a bi-functional and thermostable amylopullulanase in transgenic rice seeds leads to autohydrolysis and altered composition of starch. Molecular Breeding, 15:125-143 (SCI, Impact Factor: 2.589).

31. Chen, M.H., Huang, L.F., Li, H.M., Chen, Y.R, Yu, S.M. (2004) Signal peptide-dependent targeting of a rice α-amylase and cargo proteins to plastids and extracellular compartments of plant cells. Plant Physiology, 135: 1367-1377 (equal contribution as the first author, SCI, Impact Factor: 8.34).

* indicates corresponding author

Note: Information of Impact Factor is from Journal Citation Reports™ 2020

Research Project

-Funding from National Science Council and Ministry of Science and Technology (2008~2022)

-Young Scholar Award of Yuan Ze University (2016)

-Funding from National Science Council (2008~2020)

-Travel Grant Award from graduate student council of University of Florida, 2006, Gainesville, Florida, U.S.A. (May 2006)

-Graduate School Travel Grant Award in Plant Biology 2005, American Society of Plant Biologists, Seattle, Washington, U.S.A. (July 2005)

-Best-Presentation Award at state-wide workshop for Plant Molecular & Cellular Biology, Daytona, Florida, U.S.A. (May 2005)

Publications & Patents

Patent

US 10,041,080 B2 MODIFIED PROMOTER SEQUENCE AND APPLICATION THEREO (2018/8/7)

臺灣發明專利 , 發明第I530503號, [修飾的啟動子序列及其應用]，專利權人：元智大學；發明人：黃麗芬、陳鵬文、余淑美 (2016/04/21)

臺灣發明專利 , 發明第I792184號, [ 均質相陰離子交換膜、生物傳感膜 ]，專利權人：元智大學；發明人： 孫一明、黃麗芬、莊介寧、黃文杉 (2023/02/11)